It is also widely available in tribal belt of chhattisgarh. Winner of the standing ovation award for best powerpoint templates from presentations magazine. Calibration in tlchptlc using the michaelismenten function. Chromatography ppt powerpoint presentation, semester. Hptlc chromatogram of ritonavir 240 ngspot and lopinavir 960 ngspot standard with corresponding r. Analysis of wedelolactone, quercetin and jatamansone were performed on tlc aluminium plates precoated with silica gel 60f254 as stationary phase. This edited book is the presentation of 18 indepth national and international. Hptlcms as an efficient hyphenated technique for the rapid identification of antimicrobial compounds from propolis. Free powerpoint templates 30 slide free powerpoint. On behalf of the organizing committee, it is my pleasure to invite you to the 2018 first asia international symposium for highperformance thinlayer chromatography hptlc asia 2018 which will take place in november 2830, 2018, bangkok, thailand. Hptlcaptastaining innovative protein detection system.
Thin layer chromatography is performed on a sheet of glass, plastic, or aluminum foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminum oxide, or cellulose blotter paper. Exiso gui makes easier to extract multiple iso with a queue list and a little ftp browser. Thin layer chromatography tlc is a chromatography technique used to separate mixtures. Free powerpoint templates with alt theme for presentation. Srivastava editor the present edited book is the presentation of 18 indepth national and interna.
Various parts of the plant or the plant as a whole is used as treatment of so. Prawez alam department of pharmacognosy college of pharmacy salman bin abdulaziz university. Karthika k, jamuna s, paulsamy s abstract most of the traditional medicinal plants in india are not scientifically validated. A number of enhancements can be made to the basic method of thinlayer chromatography to automate the different steps, to increase the resolution achieved and to allow more accurate quantitative measurements. Pdf tlchptlc in biomedical applications researchgate. Chromatography equipment looks rather intimidating to anyone who has not handled it before, but on a closer look and as you get familiar with the equipment you realize that behind the network of wires, complex plumbing and circuitry is a simple machine with only a few major parts. Read a validation protocol for the hptlc standardization of herbal products. High performance thinlayer chromatography the high performance version of planar chromatography distinguishes the technique. To overcome some of the problems while using lcms or electrophoresis, highperformance thinlayer chromatography hptlc represents an interesting complementary technique 1,2,3,4. General techniques involved in phytochemical analysis international journal of advanced research in chemical science ijarcs page 27 mass is packed and the top of the percolator is closed. Tweet and share to your freinds now, well be happy.
A very good example of such thing is the green plants as they have a mixture of distinct pigments. High performance thin layer chromatography hptlc and. Introduction to high performance liquid chromatography and its parts. If you add hyperlinks to powerpoint presentations or ppt templates but want to blend the link color to your corporate identity colors or other colors used in your powerpoint templates, then you can do it.
Hptlc asia 2018 highperformance thinlayer chromatography. If so, share your ppt presentation slides online with. Highperformance thin layer chromatographic hptlc method for the quantitative estimation of wedelolactone, quercetin and jatamansone in polyherbal formulation. In this article we will see how to change the default link color in powerpoint presentations from blue to any. Part of new models of care t21 transformational initiative. Content content introduction principle instrumentation difference between tlc and hptlc steps involving in hptlc factors influencing separation and resolution of spots applications of hptlc 2 3. Hptlc study4,5 of methanolic extracts of the individual ingredients, inhouse formulation and marketed formulation were carried out along with the different marker compounds corresponding to the active ingredients to ensure the presence of active ingredients in all the formulations. For normal chromatography, solvent should be nonpolar and volatile. Hptlc comprises the use of chromatographic layers of utmost separation efficiency and the employment of stateoftheart instrumentation for all steps in the procedure. Chromatography is a physical process of separation in which the components to be separated are distributed between 2. It is also known as planar chromatography or flatbed chromatography. Hptlc take place in high speed capillary flow rang of the mobile phase, there are three main step in hptlc 1 sample to analyzed to chromatogram layer volume precision and suitable position are achieved by use of suitable instrument. Application of lc and hptlcdensitometry for the simultaneous determination of benazepril hydrochloride and hydrochlorothiazide the separation was carried out on merck hptlc aluminum sheets of silica gel 60 f254, using ethyl acetatemethanolchloroform 10.
Learn about how chemicals can be separated based on polarity through thin layer chromatography tlc. It is a major advancement of tlc principle with short time duration and better resolution. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that. Welcome to the 1st asia international symposium for highperformance thinlayer chromatography. General techniques involved in phytochemical analysis. Today chromatographic technique have little to do with the separation of color. Highperformance thinlayer chromatography wikipedia. I have a powerpoint presentation, i want to call that ppt file by clicking anchor tag with in html page, when i click on link it will open presentation but always in design mode i want to open it in slide show mode, i tried with all extensions ppt, pps, pptx, ppsx etc, although i am using office 2007 and windows xp2 with internet explorer. Focus on unhealthy behaviors to decrease risk for chronic conditions or as part of treatment for existing conditions. The proposed hptlc method provide a good resolution of quercetin from other constituents present in hydroalcoholic extract of dried flowers ofn. The book is organized in four chapters comprising of 18 contributions from experts of international repute. Few materials look homogenous but in real they are the combination of distinct substances. Thinlayer chromatography tlc is a chromatography technique used to separate nonvolatile.
Hptlc is an analytical technique based on tlc, but with enhancements intended to increase the resolution of the compounds to be separated and to allow quantitative analysis of the compounds. Ppt hptlc powerpoint presentation, free download id. Here is a reference to all the botanical species in the ahpa botanical identity references compendium which are marked as containing hptlc data. Tlc and hptlc fingerprint profiles of different bioactive components from the tuber of solena amplexicaulis. Hptlc is the only single test that allows for 100%. Hptlc is the primary chemical test for identification of all pharmacopoeia. The peak purity of quercetin was assessed by comparing the spectra at peak start, peak apex and peak end positions of the spot. Hptlcms as an efficient hyphenated technique for the. Introduction of hptlc hptlc is the improved method of tlc which utilizes the conventional technique of tlc in more optimized way. The analysis of nondenatured proteins, especially proteoforms, is still challenging due to their multitude of structural and physicochemical characteristics.
High performance thin layer chromatogarphyhptlc is the property of its rightful owner. Argemone mexicana mexican poppy is a species of poppy found in mexico and now widely naturalized in many parts of the world. Apr 26, 2020 chromatography ppt powerpoint presentation, semester, engineering chemistry class 11 notes edurev is made by best teachers of class 11. Download as ppt, pdf, txt or read online from scribd.
Hptlc is the only single test that allows for 100% botanical ingredient identification. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. Simply download our personal license alt theme powerpoint templates at no cost to you. This document is highly rated by class 11 students and has been viewed 1127 times. Application to the determination of acteoside in leaves of plantago palmata hook. Highperformance thinlayer chromatography hptlc is an enhanced form of thinlayer chromatography tlc. Hptlc tlc development chamber less amount of mobile phase more amount of mobile phase sample spotting auto sample manual spotting scanning visiblefluorosscanner scan the entire chromatogram not possible. Development of quantitative hptlc methods for dolutegravir. Thinlayer chromatography, in analytical chemistry, technique for separating dissolved chemical substances by virtue of their differential migration over glass plates or plastic sheets coated with a thin layer of a finely ground adsorbent, such as silica gel or alumina, that is mixed with a binder. Hptlc ppt free download as powerpoint presentation. With advances in technology in recent years, tlc has become more sophisticated and with the development of high performance thin layer chromatography hptlc, has the potential to provide both qualitative and quantitative data.
Powerpoint presentation open with html link in slide show. Hptlc ahpa botanical identity references compendium. The book highlights hptlc system as a versatile modern analytical technique with reference to its excellent automation, optimization, multidimensional applications. Theres a contact link on each page of the pptools site. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect. An extension of tlc is highperformance thin layer chromatography hptlc is robust, simplest, rapid, and efficient tool in quantitative analysis of compounds. The principle hptlc takes place in highspeed capillary flow range of the mobile phase.
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